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UV - VIS SPECTROPHOTOMETER
Spectrophotometer Model NanoSpec 1 NanoSpec 2 UV NanoSpec Double
Optical System Single Beam (1200 Lines/mm Grating)

Scanning UV-VIS Spectrophotometer

Double Beam (1200 Lines/mm Grating)
Wavelength Range 190 to 1100 nm 190 to 1100 nm 190 to 1100 nm
Bandwidth 1.0 nm 2 nm 0.5/1.0/2.0/4.0 nm (Optional, must be told by ordering)
Wavelength Accuracy ± 0.3 nm ± 0.05 nm ± 0.3 nm
Wavelength Repeatability 0.2 nm 0.2 nm 0.2 nm
Photometric Accuracy ± 0.3%T Auto ± 0.3%T
Photometric Repeatability 0.2%T ± 0.3%T 0.2%T
Photometric Range -0.3-3A, 0-200%T, 0-9999C 0.2%T -0.3-3A, 0-200%T, 0-9999C
Stability ± 0.002A/h @ 500nm 0-200%T, -0.3-3.0A, 0-9999C ± 0.002A/h @ 500 nm
Baseline Flatness ± 0.001A/h ± 0.002A/h @ 500nm ± 0.001A/h
Noise ± 0.001A/h ± 0.015A ± 0.001A/h
Stray Light ≤0.05%T @220nm, 360nm ≤0.05%T ≤0.05%T @220 nm, 360 nm
Data Output Port USB USB USB
Printer Output Port Parallel Port Parallel Port Parallel Port
Display 320x240 Dots LCD 128x64 Dots LCD 320x240 Dots LCD
Light source Deuterium (D2) & Tungsten (W) Halogen Lamps Deuterium (D2) & Tungsten (W) Halogen Lamps Deuterium (D2) & Tungsten (W) Halogen Lamps
Detector Silicon Photodiode Silicon Photodiode Silicon Photodiode
Power AC 220v / 50Hz, AC 110v / 60Hz AC 220v / 50Hz, AC 110v / 60Hz AC 220v / 50Hz, AC 110v / 60Hz

PC Control Software as Standard
Multi-Wavelength
Up to 32 wavelength can be selected and multiple samples
Quantitative, Coefficient Method (Standard Curve)
Use up to 32 standards to establish standard curve and methods:
Linear fit - Linear through zero - Square fit - Segmented
DNA/Protein Test
Concentration and DNA purity are Quickly and easily calculated: Absorbance ratios 260nm/280nm with optional subtracted absorbance at 320nm.
DNA Concentration =62.9 x A260 - 36.0 x A280
Protein Concentration =1552 x A260 - 757.3 x A280

UV - VIS SPECTROPHOTOMETER

Kinetics
This mode may be used for time course scanning or reaction rate calculations. Abs. vs. time graphs are displayed on the screen in real time. Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2, 5, 10, 30 secs and 1 min. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation.
Wavelength Scanning
The wavelength scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm, and Hi, Medium and Low scan speeds are available. Scan speeds vary from 100 to 23000 nm/min. Wavelengths are scanned from high to low so that the instrument waits at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final scan.

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